Abstract: This work investigates the effect of poly hydroxy materials on the behavior of silver nano-particles (SNPs) – solution produced by laser ablation technique. Different concentrations of glycerin, glucose and sucrose dissolved in double distilled water to act as carriers to (SNPs). Laser of 20 pulses with 600 mJ, 8ns pulse duration and 532 nm are applied. Absorption spectra of SNP solution were measured by UV-VIS spectrophotometer. The rheological behavior was investigated using cone-on-plate viscometer and surface tension device. The results show that a peak of Plasmon located at 400 nm which indicate that SNPs are present. The viscosity increases as hydroxyl groups concentration increasing and with SNPS size decreasing. The surface tension decreases as concentration of hydroxyl materials increasing for glycerin and sucrose while increasing with glucose. A good confirming observed between optical and viscosity results.